AKURASI ALAT DETEKSI SODIUM DALAM URIN SECARA OTOMATIS DENGAN METODE ELECTRODA SELEKSI ION KERING
Article Sidebar
Read Counter : 915
Download : 587
Main Article Content
Abstract
Inductively Couple Plasma (ICP) is a standard method to detect urinary sodium. However, the urinary sodium analysis method has a limitation in which the process to free sodium from molecules is not digest thoroughly. Ionic Selected Electrode (ISE) method is the better option. A New authomatic instrument Na-K-Cl automatic digital analyser that is “Spotchem EL 1520” using ISE method (dry ISE) was developed but the application to detect urinary sodium has not yet done. The purpose of this trial was to test the accuracy of the instrument in detecting urinary sodium. A number of 100 people aged 20-64 years participated in the trial by collecting around 50 mL fresh urine. Urinary sodium was analysed by ICP method in chemical laboratory and by ISE method using the digital analyser instrument. A number of 6 out of 100 urine sample did not meet a minimum volume requirement to be analysed by ICP method but it was able to be analysed using ISE method. The instrument was able to detect urinary sodium more than 132 (SD:77,81) mmol/L compared to Laboratory ICP method 79.38 (SD: 47,50) mmol/L. The Sensitivity of the instrument to detect sodium in urine was 97.6% (95% CI : 87.1-99.9%) and the Specivicity was 58 % (95% CI: 44.7-71.9). The trial also analysed urinary creatinine with aution instrument. A number of 10 urine sample was analysed by private clinical laboratory as quality control. Aution is semi quantitave digital analyser and the result was inline with quantitative (controlled sample). The automatic digital analyser can be used as alternative for conventional analyses of urinary sodium.
ABSTRAK
Metode deteksi konsentrasi sodium dapat dilakukan dengan metode ICP atau ISE. Saat ini telah dikembangkan alat deteksi sodium metode ISE kering yang dapat diterapkan pada sampel serum maupun urin. Uji coba alat dilakukan di laboratorium terpadu Badan Litbang Kesehatan di Bogor. Sebanyak 100 orang partisipan terlibat dalam uji coba. Partisipan adalah penduduk di sekitar kantor dan honorer penelitian kohor tumbuh kembang di Bogor. Partisipan diminta specimen urin di laboratorium dan diperiksa kadarnya pada hari itu juga. Dua alat yang diuji coba adalah Na-K-Cl digital analyzer, (Spotchem EL 1520, arkray dengan metode ISE kering) dan creatinine aution, semi kuantitatif (arkray). Keduanya menggunakan urin sebagai specimen. Uji coba dimulai dengan pemeriksaan deteksi kreatinin karena pemeriksaan harus kurang dari 24 jam. Sebanyak 50 sampel diperiksa berurutan tanpa jeda, kemudian diteruskan 50 spesimen lainnya. Kesepakatan supervisor, diperiksakan 10 specimen diperiksa di laboratorium swasta yang ada di Bogor sebagai pembanding. Ujicoba alat Na-K-Cl analyzer dengan 100 specimen urin dilakukan tanpa jeda. Pembanding hasil periksa alat ini adalah pemeriksaan urin metode ICP di laboratorium terpadu dan dikerjakan oleh analis kimia yang berpengalaman. Dari segi proses, kedua alat tidak ditemukan masalah saat ujicoba. Kedua alat ini digunakan untuk memeriksa 25 spesimen per hari. Hasil kadar kreatinin setelah dibandingkan dengan hasil periksa di laboratorium swasta secara grafik bagus walaupun dengan nilai absolut berbeda. Uji statistik menunjukkan Se 97,6 dan Sp 58. Hasil deteksi dengan alat ini lebih sensitive dibandingkan dengan laboratorium.
Kata kunci: Inductively Couple Plasma ( ICP), Ionic Selected Electrode (ISE), sodium urin
Downloads
Article Details
Authors who publish with Gizi Indonesia (Journal of The Indonesian Nutrition Association) agree to the following terms:
- Authors retain copyright and grant Gizi Indonesia (Journal of The Indonesian Nutrition Association) right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to remix, adapt, build upon the work non-commercially with an acknowledgement of the work's authorship and initial publication in Gizi Indonesia (Journal of The Indonesian Nutrition Association).
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar Tahun 2013. Badan Litbang Kesehatan 2014
Cappuccio FP, Simon Capewell S, Lincoln P chief, Klim McPherson K. Policy options to reduce population salt intake. BMJ 2011;343. crossref
He FJ, Campbell NRC, MacGregor GA. Reducing salt intake to prevent hypertension and cardiovasculardisease. Rev Panam Salud Publica. 2012;32(4):293–300.
Hawkes C, Webster J.National Approaches to Monitoring Population Salt Intake: A Trade-Off between Accuracy and Practicality? PLoS ONE 2012;7(10):e46727. crossref
Campbell NRC, Dary O, Cappuccio FP, Neufeld LM, Harding KB, Zimmermann MB. Need for coordinated programs to improve global health by optimizing salt and iodine intake. Rev Panam Salud Publica. 2012;32(4):281–6
MedlinPlus. Sodium urin test. US National Library of Medicine, National Institute of Health. URL://www.nlm.nih.gov/medlineplus/ency/article/003599.htmdiakses 20 Juni 2016
O`Donnel M, Mente A, Yusuf S. Sodium Intake and Cardiovascular Health. CircRest2015; 116; 1046-1057. crossref
Samsudin M, Nurcahyani YD, Ihsan N. Dampak intervensi garam beriodium berbagai dosis terhadap status iodium dan fungsi tiroid normal pada anak sekolah dasar. Media Gizi Mikro Indonesia 2016;8(1):1-16
WHO/PAHO. Regional expert group for cardiovascular disease prevention through population wide dietary salt reduction. Subgroup for research and surveillance. Protocol for population level Sodium determination in 24 hour urine sample. Washington DC. WHO/PAHO 2010
WHO. Guideline: Sodium intake for adults and children. Geneva, World Health Organization (WHO), 2012
Day NE, McKeown N, Wong MY, Welch A, and Binghan S. Epidemiological assessment of diet: a comparison of a 7-day dairy with a food frequency using urinary markers of nitrogen, potassium and sodium. Intl J Epid 2001;30:309-317
Anonious-Hidrolology Project. How to measure sodium. Training Module number WQ-44. World Bank and The Netherlands. New Delhi, India 2000.
Intersalt Cooperative Research Group. Intersalt: an international study of electrolyte excretion and blood pressure. Results for 24 hour urinary sodium and potassium excretion BMJ 1988;297:319-328