THE EFFECT OF BANANA-BASED ANALOG RICE ON KIDNEY HISTOPATHOLOGY IN RATS FED A HIGH FAT AND FRUCTOSE DIET
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Abstract
High Fat and Fructose Diet (HFFD) can cause hypercholesterolemia and kidney damage, which may be reduced by banana-based rice analogs due to low glycemic index, high fiber, pectin, and phenolic. The aim of this study is to assess the effect of banana-based analogue rice on kidney histopathological features in HFFD-fed rats (Rattus norvegicus). This study is a true experimental post-test only design on 24 male rats randomly devided into negative control (CN) and HFFD groups for 14 days. HFFD-fed rats group then received standart pellets (CP), white rice pellets (WR), and banana-based analog rice pelletes (BAR) for 21 days. Kidneys were examined microscopically (400x) for inflammatory cell infiltration, edema, and necrosis in the glomerulus and renal tubules. This study showed the average kidney damage scores from highest were: WR group (5.4), CP group (5.3), BAR group (3.2), and CN group (2.5). Then One-Way ANOVA and Post-Hoc Bonferroni test showed a significant difference between BAR group with CP and WR groups. Banana-based analog rice pellet improved kidney condition in rats, which kidney damage scores in the BAR group being 60 percent lower than CP and WR groups. Future studies should consider serial termination and necropsy to evaluate kidney demage progression.
Keywords: banana analog rice, dyslipidemia, HFFD, kidney histopathology
ABSTRAK
Diet tinggi lemak dan fruktosa (HFFD) dapat menyebabkan hiperkolesterolemia hingga kerusakan ginjal, yang dapat dihambat dengan beras analog berbasis pisang karena memiliki indeks glikemik rendah, tinggi serat, pektin dan fenolik. Penelitian ini bertujuan menilai efek beras analog berbasis pisang terhadap gambaran histopatologi ginjal pada tikus (Rattus norvegicus) dengan HFFD. Penelitian ini berdesain true experimental dengan post-test only pada 24 tikus jantan (Rattus norvegicus), yang secara acak dikelompokkan dalam kelompok kontrol negatif (CN) dan perlakuan HFFD selama 14 hari. Kelompok dengan HFFD selanjutnya dibagi dan menerima pelet standar (CP), pelet beras putih (WR), dan pelet beras analog berbasis pisang (BAR) selama 21 hari. Kemudian ginjal diamati menggunakan mikroskop (400x) untuk menilai infiltrasi sel inflamasi, edema, dan nekrosis pada glomerulus serta tubulus ginjal. Penelitian ini menunjukkan rerata kerusakan ginjal dari yang terberat adalah kelompok WR (5,4); kelompok CP (5,3), kelompok BAR (3,2); kelompok CN (2,5). Uji One-Way ANOVA dan Post-Hoc Bonferroni menunjukkan perbedaan signifikan antara kelompok BAR dengan kelompok CP dan WR. Pemberian pelet beras analog berbasis pisang memberikan perbaikan pada ginjal tikus dengan nilai kerusakan ginjal kelompok BAR lebih rendah 60 persen dibandingkan dengan kelompok CP dan WR. Penelitian selanjutnya disarankan melakukan terminasi dan nekropsi secara serial guna mengevaluasi progresivitas keruskaan ginjal.
Kata kunci: beras analog pisang, dislipidemia, HFFD, histopatologi ginjal
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